DNA

Part:BBa_K315018:Design

Designed by: Brianna Pearson   Group: iGEM10_Davidson-MissouriW   (2010-07-09)


pTet-loxP-RBS-RFP-loxBri(F)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

I used a moderate promoter and a oligos I designed with three mutations in order to test the interaction between variant lox sites.


Source

GCAT-alog.

References